eclipse ts2 confocal microscope Search Results


mab ts2 16  (Thermo Fisher)
99
Thermo Fisher mab ts2 16
Mab Ts2 16, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/Saponin/pm09628888-76-45-94
Average 99 stars, based on 1 article reviews
mab ts2 16 - by Bioz Stars, 2026-09
99/100 stars
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confocal microscope  (Nikon)
99
Nikon confocal microscope
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/Objectives/pm40357751-171-7-9
Average 99 stars, based on 1 article reviews
confocal microscope - by Bioz Stars, 2026-09
99/100 stars
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confocal laser scanning microscope  (Olympus)
99
Olympus confocal laser scanning microscope
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Confocal Laser Scanning Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/FV3000+Confocal+Laser+Scanning+Microscope/pm41268785-243-12-16
Average 99 stars, based on 1 article reviews
confocal laser scanning microscope - by Bioz Stars, 2026-09
99/100 stars
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autoflow water jacket co2 incubator  (NuAire)
95
NuAire autoflow water jacket co2 incubator
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Autoflow Water Jacket Co2 Incubator, supplied by NuAire, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/AutoFlow+Water+Jacket+CO2+Incubator/custom%40nu-4750%4037115798
Average 95 stars, based on 1 article reviews
autoflow water jacket co2 incubator - by Bioz Stars, 2026-09
95/100 stars
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ckx 53 inverted microscope  (Olympus)
99
Olympus ckx 53 inverted microscope
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Ckx 53 Inverted Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/CKX53+Cell+Culture+Microscope/bio_rxiv__2024__10__15__618593-93-12-11
Average 99 stars, based on 1 article reviews
ckx 53 inverted microscope - by Bioz Stars, 2026-09
99/100 stars
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mica  (Danaher Inc)
95
Danaher Inc mica
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Mica, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/Mica/custom%40mica%4037805057
Average 95 stars, based on 1 article reviews
mica - by Bioz Stars, 2026-09
95/100 stars
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larvae  (Nikon)
99
Nikon larvae
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Larvae, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/ECLIPSE+Ti2/pm36613936-262-5-10
Average 99 stars, based on 1 article reviews
larvae - by Bioz Stars, 2026-09
99/100 stars
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pressure-volume catheter  (Millar Inc)
90
Millar Inc pressure-volume catheter
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Pressure Volume Catheter, supplied by Millar Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/microtip+catheter+transducer+spr+839/pmc11466196__jciinsight___9___181877___s130-17-84-87
Average 90 stars, based on 1 article reviews
pressure-volume catheter - by Bioz Stars, 2026-09
90/100 stars
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health  (Bio-Rad)
99
Bio-Rad health
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Health, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/Image+Lab+Software/pm41794726-646-89-91
Average 99 stars, based on 1 article reviews
health - by Bioz Stars, 2026-09
99/100 stars
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ff01 539 30  (IDEX)
86
IDEX ff01 539 30
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Ff01 539 30, supplied by IDEX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/bandpass+filter/pm39700014-274-114-111
Average 86 stars, based on 1 article reviews
ff01 539 30 - by Bioz Stars, 2026-09
86/100 stars
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resource source identifier imaris 8 2  (Oxford Instruments)
99
Oxford Instruments resource source identifier imaris 8 2
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
Resource Source Identifier Imaris 8 2, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ts2+confocal+microscope/Imaris/pm39700014-274-2-5
Average 99 stars, based on 1 article reviews
resource source identifier imaris 8 2 - by Bioz Stars, 2026-09
99/100 stars
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ff555 di03 bandpass filters semrock ff01 417 60  (IDEX)
86
IDEX ff555 di03 bandpass filters semrock ff01 417 60
Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
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Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron microscope show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.

Journal: Biology open

Article Title: Primary human neutrophils and monocytes migrate along endothelial cell boundaries to optimize search efficiency under static in vitro conditions.

doi: 10.1242/bio.061704

Figure Lengend Snippet: Fig. 1. Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown (N=3, n=196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown (N=3, n=12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron microscope show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.

Article Snippet: Cell migration was imaged using an inverted confocal microscope (Nikon, Eclipse Ts2) with a digital camera (Nikon DS-Fi2), using a 20× objective at 37°C and 5% CO2 with a frame interval of 60 s. Using multi-frame imaging allowed the recording ofmultiple positions per channel (i.e. technical repeats).

Techniques: Cell Culture, Immunocytochemistry, Staining, Negative Control, Migration, Fluorescence, Transmigration Assay, Derivative Assay, Microscopy